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A Data Carpentry Workshop

Tufts University Sackler School of Graduate Biomedical Sciences

Jan 26-27, 2017

9:00 am - 4:30 pm

Instructors: Sue McClatchy, Dan Gatti, Eric Ma, Bob Freeman, Brian Teague, Asli Uyar

Helpers: David LaPointe, Albert Tai, Norbert Kremer

General Information

Data Carpentry workshops are for any researcher who has data they want to analyze, and no prior computational experience is required. This hands-on workshop teaches basic concepts, skills and tools for working more effectively with data.

We will cover Introduction to the Shell and Cloud Computing, The Shell and Project Setup, Know Your Data and Variant Calling and Workflow Automation. Participants should bring their laptops and plan to participate actively. By the end of the workshop learners should be able to more effectively manage and analyze data and be able to apply the tools and approaches directly to their ongoing research.

Who: The workshop is sponsored by the graduate Immunology Program and is open primarily to graduate students, faculty and postdoctoral fellows in immunology.

Where: 145 Harrison Avenue, Boston, Massachusetts. Get directions with OpenStreetMap or Google Maps.

Requirements: Participants must bring a laptop with a Mac, Linux, or Windows operating sytem (not a tablet, Chromebook, etc.) that they have administrative privileges on. They should have a few specific software packages installed (listed below). They are also required to abide by Data Carpentry's Code of Conduct.

Contact: Please mail diana.pierce@tufts.edu for more information.


Day 1

Morning Introduction to the Shell and Cloud Computing
Afternoon The Shell and Project Setup

Day 2

Morning Know Your Data
Afternoon Variant Calling and Workflow Automation

Etherpad: http://pad.software-carpentry.org/2017-01-26-tufts.
We will use this Etherpad for chatting, taking notes, and sharing URLs and bits of code.

Surveys

Please be sure to complete these surveys before and after the workshop.

Pre-workshop Survey

Post-workshop Survey

Preliminary Schedule

Thursday, January 26

MorningIntroduction to the Shell and Cloud Computing
09:00 Workshop overview
09:30 Using cloud computing for genomics
10:00 Introduction to Unix: navigating, paths, shortcuts
10:45 Coffee/tea break
11:00 Introduction to Unix: examining files, modifying, writing
12:00 Lunch on your own
Afternoon The Shell and Project Setup
13:00 Introduction to Unix: searching and redirection
13:45 Shell scripts
14:30 Coffee/tea break
14:45 Shell scripts (cont)
15:45 Genomics planning
16:45 Wrap-up

Friday, January 27

Morning Know Your Data
09:00 Introduction to the workshop dataset
09:15 Examining data on the NCBI SRA database
09:45 QC of NGS data
10:30 Coffee/tea break
10:45 QC of NGS data (cont)
12:00 Lunch on your own
Afternoon Variant Calling and Workflow Automation
13:00 Variant calling workflow (QC, alignment, variant calling)
14:00 Automating the workflow
14:30 Coffee/tea break
14:45 Automating the workflow (cont)
16:45 Wrap-up

Syllabus

The Unix Shell

  • Files and directories
  • History and tab completion
  • Pipes and redirection
  • Looping over files
  • Creating and running shell scripts
  • Finding things
  • Reference...

Setup

To participate in a Data Carpentry workshop, you will need working copies of the described software. Please make sure to install everything (or at least to download the installers) before the start of your workshop. Participants should bring and use their own laptops to insure the proper setup of tools for an efficient workflow once you leave the workshop.

Mac users

Windows users

We maintain a list of common issues that occur during installation as a reference for instructors that may be useful on the Configuration Problems and Solutions wiki page.